Neeru Yadav, Namrata Namrata, Shardulaya Shukla, Arup Giri*
DOI: https://doi.org/10.14429/dlsj.19975
ABSTRACT. The objective of this study is to assess the changes in the antioxidant activity of milk in accordance with the season and farm locations in Haryana. The antioxidant potential of milk samples was evaluated through two different methods: the FRAP assay and the DPPH assay. The FRAP level was significantly higher during the summer season, except in the case of buffalo milk. The levels (μmol/mL) were recorded at 70.00±4.22, 60.86±5.29, and 73.69±7.87 in the summer season and 72.64±8.22, 60.42±1.32, and 57.45±1.21 in the winter season for buffalo, cow, and goat milk, respectively. On the other hand, DPPH scavenging activity showed a higher trend in the summer season, but not significantly. DPPH levels were recorded at 19.12±2.53, 16.55±1.04, and 20.73±1.51 in the summer season for buffalo, cow, and goat milk samples, respectively. The reason could be attributed to an increase in antioxidative activity as an adaptation to heat stress conditions and variations in seasonal feed. The productivity and quality of milk from dairy animals change according to climatic conditions. Notably, significant differences were observed among locations, which could be linked to differences in the feeding management of farms. So far, not much research has been conducted on this aspect in Haryana. This study will explore the correlation of animal antioxidative properties with variations in season and across different locations.
Keywords: Antioxidant; Bioactivity; Dairy; DPPH; FRAP
Citation: Neeru Yadav, Namrata Namrata, Shardulaya Shukla, Arup Giri*. (2026) Evaluation of antioxidant activity in buffalo, cow and goat milk of two different seasons in Haryana, India. Defence Life Science Journal, ISSN- 2456-379X. [IF-0.0] [Q4] Indexing: Scopus; Published: 08 January 2026)
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